Codon Optimization, Cloning and Expression of the Human Leukemia Inhibitory Factor (hLIF) in E. coli
نویسندگان
چکیده
منابع مشابه
codon optimization, cloning and expression of the human leukemia inhibitory factor (hlif) in e. coli
background: leukemia inhibitor factor (lif) is a very important pleiotropic cytokine which belongs to interleukin-6 (il-6) family. lif exerts multiple effects on different types of cells and tissues with numerous regulatory effects in vivo and in vitro. it is a lymphoid factor, which performs a number of activities including cholinergic neuron differentiation, control of stem cell pluripotency...
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Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...
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The plasmid pALCA1SIFN containing cDNA that encodes the human interferon a-2b was obtained from the ATCC(no. 531667). In this system the expression of the gene is under the control of an alcA promoter. alcA p is a specific promoter for expression of different genes in Aspergillusfilamentous. In this plasmid the coding region of IFN?-2b is preceded by the coding region of a synthetic signal ...
متن کاملcloning, expression, and functional characterization of in-house prepared human leukemia inhibitory factor
objective: leukemia inhibitory factor (lif) plays important roles in cellular proliferation, growth promotion and differentiation of various types of target cells. in addition, lif influences bone metabolism, cachexia, neural development, embryogenesis and inflammation. human lif (hlif) is an essential growth factor for the maintenance of mouse embryonic stem cells (escs) and induced pluripoten...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2012
ISSN: 1728-3043,2322-2921
DOI: 10.5812/ijb.9229